Hotspot

ccsA-ndhD

SSC · 118728-118945 · 218 bp

Back to Hydriastele

Pi 0.0139

MarkerSeek score 61.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 218
variable_sites: 3
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACGAATTCGGCACTTATAGCT	TCAGTTGACAGGGTACAAGC	42.9	50.0	57.5	57.5	384-386 bp	1.0	65.1
ccsA-ndhD_p2	ACGAATTCGGCACTTATAGCT	CAGTTGACAGGGTACAAGCT	42.9	50.0	57.5	57.4	383-385 bp	1.0	65.0
ccsA-ndhD_p3	ACGAATTCGGCACTTATAGC	TCAGTTGACAGGGTACAAGC	45.0	50.0	56.0	57.5	384-386 bp	1.0	61.0
ccsA-ndhD_p4	ACGAATTCGGCACTTATAGCT	ATCAGTTGACAGGGTACAAGC	42.9	47.6	57.5	57.9	385-387 bp	1.0	60.9
ccsA-ndhD_p5	ACGAATTCGGCACTTATAGC	CAGTTGACAGGGTACAAGCT	45.0	50.0	56.0	57.4	383-385 bp	1.0	60.9