Hotspot

rbcL-accD

LSC · 58924-59727 · 804 bp

Back to Hydriastele

Pi 0.00373

MarkerSeek score 60.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 3
pi: 0.00373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	GGGTTCTTCACTTCCACTGG	50.0	55.0	57.9	58.1	1013-1025 bp	1.0	78.4
rbcL-accD_p2	TAGCAAATGGAGCCCTGAAC	GGGTTCTTCACTTCCACTGG	50.0	55.0	57.9	58.1	1015-1027 bp	1.0	78.4
rbcL-accD_p3	TGAAGCTAGCAAATGGAGCC	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.2	58.1	1021-1033 bp	1.0	77.5
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.3	58.1	1024-1036 bp	1.0	77.3
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.3	58.1	1025-1037 bp	1.0	77.3