Hotspot

petN-psbM

LSC · 30201-30640 · 440 bp

Back to Hydriastele

Pi 0.0137

MarkerSeek score 63.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 6
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	595-596 bp	1.0	68.2
petN-psbM_p2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.7	57.6	590-591 bp	1.0	67.2
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	540-541 bp	1.0	67.0
petN-psbM_p4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.6	57.6	599-600 bp	1.0	65.2
petN-psbM_p5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.6	57.6	598-599 bp	1.0	64.5