Hotspot

atpF-atpH

LSC · 13890-14416 · 527 bp

Back to Hydriastele

Pi 0.00384

MarkerSeek score 60.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 2
pi: 0.00384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	645-650 bp	1.0	79.7
atpF-atpH_p2	CAGTGACCCAAGGAAAGGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.3	58.0	617-622 bp	1.0	75.5
atpF-atpH_p3	CAGTGACCCAAGGAAAGGAAA	ATTTATGGACTGGTCGTGGC	47.6	50.0	58.1	58.0	617-622 bp	1.0	72.3
atpF-atpH_p4	GTGACCCAAGGAAAGGAAAGA	ATTTATGGACTGGTCGTGGC	47.6	50.0	57.8	58.0	615-620 bp	1.0	70.9
atpF-atpH_p5	CCAGTGACCCAAGGAAAGGA	ATTTATGGACTGGTCGTGGC	55.0	50.0	59.2	58.0	618-623 bp	1.0	70.9