Hotspot

ycf1

SSC · 127050-132752 · 5703 bp

Back to Hydrangea

Pi 0.0243

MarkerSeek score 62.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5703
variable_sites: 682
pi: 0.0243
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCACTTCTTTTATTTGTTCTTCG	GCAACTGTTTCAAGCAAATG	33.3	40.0	55.9	54.6	174-180 bp	1.0	48.0
ycf1_p2	TCCACTTCTTTTATTTGTTCTTCGG	GCAACTGTTTCAAGCAAATG	36.0	40.0	58.0	54.6	174-180 bp	0.928571	45.1
ycf1_p3	CCACTTCTTTTATTTGTTCTTCGG	GCAACTGTTTCAAGCAAATG	37.5	40.0	56.8	54.6	173-179 bp	0.928571	45.1
ycf1_p4	CCACTTCTTTTATTTGTTCTTCGGT	GCAACTGTTTCAAGCAAATG	36.0	40.0	58.3	54.6	173-179 bp	0.928571	45.1
ycf1_p5	CACTTCTTTTATTTGTTCTTCGGT	GCAACTGTTTCAAGCAAATG	33.3	40.0	56.1	54.6	172-178 bp	0.928571	45.1