Hotspot

rpl32-trnL-UAG

SSC · 116431-117360 · 930 bp

Back to Hydrangea

Pi 0.0317
MarkerSeek score 73.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
930
variable_sites
150
pi
0.0317
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTCCTTGAGCTTGGCCAAT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1583-1644 bp 0.714286 79.4
rpl32-trnL-UAG_p2 TTTCCTTGAGCTTGGCCAAT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 1584-1645 bp 0.714286 79.4
rpl32-trnL-UAG_p3 TTCCTTGAGCTTGGCCAATT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1582-1643 bp 0.678571 78.0
rpl32-trnL-UAG_p4 TTCCTTGAGCTTGGCCAATT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 1583-1644 bp 0.678571 77.9
rpl32-trnL-UAG_p5 TTCCTTGAGCTTGGCCAATT GCACTTGGAACGGTTATTGC 45.0 50.0 58.0 58.0 2046-2106 bp 0.678571 76.3