Hotspot

petA-psbJ

LSC · 65453-66440 · 988 bp

Back to Hydrangea

Pi 0.0349

MarkerSeek score 74.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 140
pi: 0.0349
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCAGAGCTATCGCCCCAATA	GGAAGTAGGGTAAATGGCCG	50.0	55.0	58.0	58.0	1676-1708 bp	1.0	91.1
petA-psbJ_p2	TCAGAGCTATCGCCCCAATA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2111-2159 bp	1.0	90.3
petA-psbJ_p3	TCAGAGCTATCGCCCCAATA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2110-2158 bp	1.0	90.3
petA-psbJ_p4	TCAGAGCTATCGCCCCAATA	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1907-1939 bp	0.964286	88.7
petA-psbJ_p5	TCAGAGCTATCGCCCCAATA	ACGACACAATCAAACCCGAA	50.0	45.0	58.0	58.0	1910-1942 bp	0.785714	79.6