Hotspot

trnG-GCC-trnM-CAU

LSC · 38004-38198 · 195 bp

Back to Hydrangea

Pi 0.04

MarkerSeek score 74.60

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 195
variable_sites: 38
pi: 0.04
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	TTGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	326-349 bp	1.0	90.8
trnG-GCC-trnM-CAU_p2	TTGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	290-313 bp	1.0	90.7
trnG-GCC-trnM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	297-320 bp	1.0	90.4
trnG-GCC-trnM-CAU_p4	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	261-284 bp	1.0	90.3
trnG-GCC-trnM-CAU_p5	TTGCGGATATGGTCGAATGG	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	284-307 bp	1.0	90.2