Hotspot

atpH-atpI

LSC · 14408-15541 · 1134 bp

Back to Hydrangea

Pi 0.02

MarkerSeek score 83.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 131
pi: 0.02
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CAAGCGGTATCCAAGCTCTT	45.0	50.0	58.0	58.0	1011-1303 bp	1.0	91.5
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CAAGCGGTATCCAAGCTCTT	50.0	50.0	57.9	58.0	1067-1359 bp	1.0	90.9
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CAAGCGGTATCCAAGCTCTT	45.0	50.0	58.2	58.0	1012-1304 bp	1.0	90.3
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CAAGCGGTATCCAAGCTCTT	55.0	50.0	58.2	58.0	1071-1363 bp	1.0	90.2
atpH-atpI_p5	TACCTTCTACAGCTTGGCCT	CAAGCGGTATCCAAGCTCTT	50.0	50.0	58.0	58.0	1094-1376 bp	0.928571	88.4