Hotspot

trnS-GCU-trnG-UCC

LSC · 9117-9849 · 733 bp

Back to Hydrangea

Pi 0.0282

MarkerSeek score 75.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 111
pi: 0.0282
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AGGGGGTTAATAGAACGAATCAC	50.0	43.5	58.0	58.0	810-852 bp	1.0	67.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AGGGGGTTAATAGAACGAATCAC	50.0	43.5	58.0	58.0	809-851 bp	1.0	67.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AGGGGGTTAATAGAACGAATCAC	50.0	43.5	58.2	58.0	864-906 bp	1.0	66.5
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GGGGGTTAATAGAACGAATCACA	50.0	43.5	58.0	58.2	809-851 bp	1.0	66.1
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	GGGGGTTAATAGAACGAATCACA	50.0	43.5	58.0	58.2	808-850 bp	1.0	66.1