Hotspot

rpl22

LSC · 91047-91481 · 435 bp

Back to Hydnocarpus

Pi 0.0322

MarkerSeek score 59.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 435
variable_sites: 14
pi: 0.0322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CGCCCTGCAATTTGTACTTG	GCAAAAGAAGGGAAATCGGC	50.0	50.0	58.0	58.0	1731 bp	1.0	81.4
rpl22_p2	CGCCCTGCAATTTGTACTTG	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.0	58.0	1657 bp	1.0	81.4
rpl22_p3	CGCCCTGCAATTTGTACTTG	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.0	58.0	1656 bp	1.0	81.4
rpl22_p4	CGTGCAATTCCTTCAACACC	ACAGTCGGACAAGTAGGGAA	50.0	50.0	57.9	58.0	1341 bp	1.0	81.2
rpl22_p5	CGTGCAATTCCTTCAACACC	GCAAAAGAAGGGAAATCGGC	50.0	50.0	57.9	58.0	1416 bp	1.0	81.1