Hotspot

rpl36-rps8

LSC · 87085-87563 · 479 bp

Back to Hydnocarpus

Pi 0.0334

MarkerSeek score 61.90

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 16
pi: 0.0334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TGTACCTCTTTTTGTGCCCC	GCGGGATGGGGATTGTAATT	50.0	50.0	58.0	58.0	1026 bp	0.5	59.9
rpl36-rps8_p2	ATAACTTCGGCTCGTTGCAT	GCGGGATGGGGATTGTAATT	45.0	50.0	58.0	58.0	1103 bp	0.5	59.8
rpl36-rps8_p3	ATTCGTCCCCGTCTACGTAT	GCGGGATGGGGATTGTAATT	50.0	50.0	58.0	58.0	651 bp	0.5	59.7
rpl36-rps8_p4	TCCCCGTCTACGTATCAGTC	GCGGGATGGGGATTGTAATT	55.0	50.0	58.0	58.0	646 bp	0.5	59.6
rpl36-rps8_p5	AATGCTGCATCTCTTCCGAG	GCGGGATGGGGATTGTAATT	50.0	50.0	58.0	58.0	1139 bp	0.5	59.6