Hotspot

psbN-psbH

LSC · 81579-81682 · 104 bp

Back to Hydnocarpus

Pi 0.0288

MarkerSeek score 68.40

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 104
variable_sites: 3
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	CGACTAGGGTTGCTGTTTCC	TCAACGCTTTGTGTAGCCAT	55.0	45.0	58.6	58.1	146 bp	1.0	76.3
psbN-psbH_p2	GACTAGGGTTGCTGTTTCCA	TCAACGCTTTGTGTAGCCAT	50.0	45.0	57.4	58.1	145 bp	1.0	76.3
psbN-psbH_p3	CGACTAGGGTTGCTGTTTCC	ACCCTCAACGCTTTGTGTAG	55.0	50.0	58.6	58.1	150 bp	1.0	76.1
psbN-psbH_p4	CGACTAGGGTTGCTGTTTCC	CTACCCTCAACGCTTTGTGT	55.0	50.0	58.6	58.1	152 bp	1.0	76.1
psbN-psbH_p5	TAGTTGTTGAGAGGGTTGCC	TCAACGCTTTGTGTAGCCAT	50.0	45.0	57.4	58.1	226 bp	1.0	75.7