Hotspot

rpl20-rps12

LSC · 75917-76720 · 804 bp

Back to Hydnocarpus

Pi 0.0124

MarkerSeek score 53.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 10
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTTGTTCTACGCCTCCGAG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	879 bp	1.0	79.3
rpl20-rps12_p2	GTAAGTCTTGAATGGGCCCC	CGCCGAGGAACATGTACTAG	55.0	55.0	58.2	57.9	926 bp	1.0	78.0
rpl20-rps12_p3	TTTTGTTCTACGCCTCCGAG	GCCGAGGAACATGTACTAGG	50.0	55.0	57.9	57.5	878 bp	1.0	76.2
rpl20-rps12_p4	TTTTTGTTCTACGCCTCCGA	CGCCGAGGAACATGTACTAG	45.0	55.0	57.5	57.9	880 bp	1.0	75.7
rpl20-rps12_p5	TTCTACGCCTCCGAGCTATA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.4	57.9	874 bp	1.0	75.5