Hotspot

trnP-UGG-psaJ

LSC · 73526-73953 · 428 bp

Back to Hydnocarpus

Pi 0.0234

MarkerSeek score 54.70

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 10
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CGAACTGAACTAAGAGCGCT	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.0	58.0	877 bp	1.0	81.2
trnP-UGG-psaJ_p2	GTTTTGGAGACCCACGTTCT	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.0	58.0	899 bp	1.0	81.0
trnP-UGG-psaJ_p3	CCGCGCCAATTTTCAATTCT	TGTCAACGCATCTGGGAAAA	45.0	45.0	58.0	58.0	1273 bp	0.5	59.8
trnP-UGG-psaJ_p4	CCGCGCCAATTTTCAATTCT	TTTACCCTTGGCCATGAACC	45.0	50.0	58.0	58.1	1763 bp	0.5	59.4
trnP-UGG-psaJ_p5	CCGCGCCAATTTTCAATTCT	CTTGGCCATGAACCTCCTTT	45.0	50.0	58.0	58.1	1757 bp	0.5	59.4