Hotspot

psbE-petL

LSC · 71392-72629 · 1238 bp

Back to Hydnocarpus

Pi 0.0162

MarkerSeek score 64.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 20
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCCTATCTTGCTCAGACCA	50.0	47.6	57.8	57.9	1354 bp	1.0	71.1
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGTCCTATCTTGCTCAGACCA	55.0	47.6	58.2	57.9	1349 bp	1.0	70.7
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGTCCTATCTTGCTCAGACCA	50.0	47.6	58.4	57.9	1347 bp	1.0	69.5
psbE-petL_p4	TGACGAATAACCAACCTGCA	AGTCCTATCTTGCTCAGACCA	45.0	47.6	57.4	57.9	1442 bp	1.0	67.8
psbE-petL_p5	TTCTCCTGTGCTTCCAGACA	AGTCCTATCTTGCTCAGACCA	50.0	47.6	58.6	57.9	1348 bp	1.0	67.7