Hotspot

matK-trnK-UUU

LSC · 3974-4695 · 722 bp

Back to Hydnocarpus

Pi 0.0263

MarkerSeek score 66.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 19
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TTCTCGAAAGATCAGGGGGT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	830 bp	1.0	80.1
matK-trnK-UUU_p2	TTCTCGAAAGATCAGGGGGT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	829 bp	1.0	80.1
matK-trnK-UUU_p3	TTCTCGAAAGATCAGGGGGT	TTGGGTTGCTAACTCAACGG	50.0	50.0	58.0	58.4	845 bp	1.0	78.2
matK-trnK-UUU_p4	TTCTCGAAAGATCAGGGGGT	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.0	57.5	843 bp	1.0	77.7
matK-trnK-UUU_p5	TTCTCGAAAGATCAGGGGGT	CAACGGTAGAGTACTCGGCT	50.0	55.0	58.0	58.9	831 bp	1.0	74.2