Hotspot

ndhB

Genome · 100518-102716 · 2199 bp

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Pi 0.0106

MarkerSeek score 51.20

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 2199
variable_sites: 44
pi: 0.0106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	GTTGGGTAGGTCCACAAACA	ATGCTGCCGAAAATAGTGGT	50.0	45.0	57.6	57.9	2870-2879 bp	0.75	68.3
ndhB_p2	GTTGGGTAGGTCCACAAACA	GCCGGGTCGAAATATAGCTT	50.0	50.0	57.6	57.8	2914-2923 bp	0.75	67.9
ndhB_p3	GTTGGGTAGGTCCACAAACA	TGGATGCTGCCGAAAATAGT	50.0	45.0	57.6	57.6	2873-2882 bp	0.75	66.0
ndhB_p4	AGTTGGGTAGGTCCACAAAC	ATGCTGCCGAAAATAGTGGT	50.0	45.0	57.3	57.9	2871-2880 bp	0.75	65.9
ndhB_p5	AGTTGGGTAGGTCCACAAAC	GCCGGGTCGAAATATAGCTT	50.0	50.0	57.3	57.8	2915-2924 bp	0.75	65.5