Hotspot

rpl32-rpl21

Genome · 137846-137986 · 141 bp

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Pi 0.0437

MarkerSeek score 67.80

Primer available yes

Label rpl32-rpl21

Feature Metadata

label_name: rpl32-rpl21
feature_type: igs
region: Genome
length_bp: 141
variable_sites: 12
pi: 0.0437
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-rpl21_p1	CCATCCGAAGTTTGTCCACA	AAACTGGGGGTGAACAACTC	50.0	50.0	58.1	57.9	1158-1159 bp	1.0	81.2
rpl32-rpl21_p2	CCATCCGAAGTTTGTCCACA	GAAACTGGGGGTGAACAACT	50.0	50.0	58.1	57.9	1159-1160 bp	1.0	81.2
rpl32-rpl21_p3	GAGCGACCAGTCAAAACAGA	AAACTGGGGGTGAACAACTC	50.0	50.0	58.1	57.9	613 bp	1.0	80.7
rpl32-rpl21_p4	GAGCGACCAGTCAAAACAGA	GAAACTGGGGGTGAACAACT	50.0	50.0	58.1	57.9	614 bp	1.0	80.7
rpl32-rpl21_p5	CCATCCGAAGTTTGTCCACA	TGATGTTCGTCACTTCGCTT	50.0	45.0	58.1	57.9	1116 bp	0.25	47.9