Hotspot

trnP-GGG-rpl32

Genome · 136832-137674 · 843 bp

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Pi 0.0194
MarkerSeek score 59.60
Primer available yes
Label trnP-GGG-rpl32

Feature Metadata

label_name
trnP-GGG-rpl32
feature_type
igs
region
Genome
length_bp
843
variable_sites
31
pi
0.0194
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-rpl32_p1 GAGGTCGTGGGTTCAAATCC GCAGCAAATAATGATATTTCTAAA 55.0 25.0 58.5 52.3 898-901 bp 1.0 43.1
trnP-GGG-rpl32_p2 GGAGGTCGTGGGTTCAAATC GCAGCAAATAATGATATTTCTAAA 55.0 25.0 58.5 52.3 899-902 bp 1.0 43.1
trnP-GGG-rpl32_p3 GGGTTCAAATCCCACTGCTC GCAGCAAATAATGATATTTCTAAA 55.0 25.0 58.8 52.3 890-893 bp 1.0 43.1
trnP-GGG-rpl32_p4 GGTTCAAATCCCACTGCTCC GCAGCAAATAATGATATTTCTAAA 55.0 25.0 58.8 52.3 889-892 bp 1.0 43.1
trnP-GGG-rpl32_p5 GTAGCGTACCATCTTGGGGT GCAGCAAATAATGATATTTCTAAA 55.0 25.0 59.2 52.3 922-925 bp 1.0 43.0