Hotspot

trnL-CAA-ndhB

Genome · 99529-100517 · 989 bp

Back to Huperzia

Pi 0.0187

MarkerSeek score 60.70

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: Genome
length_bp: 989
variable_sites: 35
pi: 0.0187
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	CTCGCGTGTCTACCATTTCA	GCGGGTCCACATTCATTAGT	50.0	50.0	58.0	58.0	1298-1313 bp	1.0	83.5
trnL-CAA-ndhB_p2	TTCTGATGCCCTGAAGAGGA	GCGGGTCCACATTCATTAGT	50.0	50.0	58.0	58.0	1355-1370 bp	1.0	83.1
trnL-CAA-ndhB_p3	GACTCGAACCTCCATGCTTT	GCGGGTCCACATTCATTAGT	50.0	50.0	57.9	58.0	1337-1352 bp	1.0	82.7
trnL-CAA-ndhB_p4	CTCGCGTGTCTACCATTTCA	CTTCTTGTCTTGCCTCGTGT	50.0	50.0	58.0	58.1	1400-1408 bp	0.5	61.5
trnL-CAA-ndhB_p5	CTCGCGTGTCTACCATTTCA	TTCTTGTCTTGCCTCGTGTC	50.0	50.0	58.0	58.1	1399-1407 bp	0.5	61.4