Hotspot

trnF-GAA-trnL-UAA

Genome · 39931-41425 · 1495 bp

Back to Huperzia

Pi 0.0166
MarkerSeek score 58.20
Primer available yes
Label trnF-GAA-trnL-UAA

Feature Metadata

label_name
trnF-GAA-trnL-UAA
feature_type
igs
region
Genome
length_bp
1495
variable_sites
47
pi
0.0166
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-trnL-UAA_p1 TCTACCGACTGAGCTATCCC GGGTTCGAGTCCCTCTACC 55.0 63.2 57.7 58.5 1534-1544 bp 1.0 68.7
trnF-GAA-trnL-UAA_p2 TCTACCGACTGAGCTATCCC GGTTCGAGTCCCTCTACCC 55.0 63.2 57.7 58.5 1533-1543 bp 1.0 68.7
trnF-GAA-trnL-UAA_p3 TCTACCGACTGAGCTATCCC GTTCGAGTCCCTCTACCCC 55.0 63.2 57.7 58.5 1532-1542 bp 1.0 68.7
trnF-GAA-trnL-UAA_p4 ATCCTCTGCTCTACCGACTG GGTTCGAGTCCCTCTACCC 55.0 63.2 58.3 58.5 1542-1552 bp 1.0 68.4
trnF-GAA-trnL-UAA_p5 ATCCTCTGCTCTACCGACTG GTTCGAGTCCCTCTACCCC 55.0 63.2 58.3 58.5 1541-1551 bp 1.0 68.4