Hotspot

psbJ-petA

Genome · 25094-26440 · 1347 bp

Back to Huperzia

Pi 0.0139
MarkerSeek score 56.10
Primer available yes
Label psbJ-petA

Feature Metadata

label_name
psbJ-petA
feature_type
igs
region
Genome
length_bp
1347
variable_sites
37
pi
0.0139
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbJ-petA_p1 GGTTCATACTCCGGATTGGG TCCATTACGTGTTCAAGGTCT 55.0 42.9 57.7 57.0 1498 bp 1.0 63.6
psbJ-petA_p2 GGTTCATACTCCGGATTGGG ACGTGTTCAAGGTCTTTTGT 55.0 40.0 57.7 55.7 1492 bp 1.0 61.8
psbJ-petA_p3 GGTTCATACTCCGGATTGGG TCCATTACGTGTTCAAGGTCTT 55.0 40.9 57.7 57.7 1498 bp 1.0 61.2
psbJ-petA_p4 GGTTCATACTCCGGATTGGG ACGTGTTCAAGGTCTTTTGTT 55.0 38.1 57.7 56.5 1492 bp 1.0 60.1
psbJ-petA_p5 GGTTCATACTCCGGATTGGG ATCCATTACGTGTTCAAGGTCT 55.0 40.9 57.7 57.4 1499 bp 1.0 59.5