Hotspot

rps12-rpl20

Genome · 17692-19975 · 2284 bp

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Pi 0.0144

MarkerSeek score 63.70

Primer available yes

Label rps12-rpl20

Feature Metadata

label_name: rps12-rpl20
feature_type: igs
region: Genome
length_bp: 2284
variable_sites: 60
pi: 0.0144
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	GGAATCAAAGACAGCCGGTA	CTGGTTACCGGTCCGAAAAA	50.0	50.0	57.6	58.1	2461-2484 bp	1.0	78.7
rps12-rpl20_p2	GGAATCAAAGACAGCCGGTA	ACGTTTACGAGCTACAGAGC	50.0	50.0	57.6	57.7	2389-2412 bp	1.0	77.7
rps12-rpl20_p3	GGAATCAAAGACAGCCGGTA	TATTTGCTGGTTACCGGTCC	50.0	50.0	57.6	57.6	2467-2490 bp	1.0	76.4
rps12-rpl20_p4	TAGGAATCAAAGACAGCCGG	CTGGTTACCGGTCCGAAAAA	50.0	50.0	57.3	58.1	2463-2486 bp	1.0	76.3
rps12-rpl20_p5	ATAGAACCAAATCCCCTGCC	CTGGTTACCGGTCCGAAAAA	50.0	50.0	57.2	58.1	2437-2460 bp	1.0	75.8