Hotspot

psbN-psbT

Genome · 12402-12568 · 167 bp

Back to Huperzia

Pi 0.018

MarkerSeek score 65.10

Primer available yes

Label psbN-psbT

Feature Metadata

label_name: psbN-psbT
feature_type: igs
region: Genome
length_bp: 167
variable_sites: 6
pi: 0.018
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbT_p1	TGGTTATGCCTTGTACACCG	TTTCGCTATTTCTTCCCGGG	50.0	50.0	57.9	58.0	298 bp	1.0	80.5
psbN-psbT_p2	ACCCTGGTCGCTATCTTCAT	TTTCGCTATTTCTTCCCGGG	50.0	50.0	58.2	58.0	342 bp	1.0	79.5
psbN-psbT_p3	TGGTTATGCCTTGTACACCG	AACCACCCAAAGTTCCAAGT	50.0	45.0	57.9	57.5	278 bp	1.0	76.6
psbN-psbT_p4	ACCCTGGTCGCTATCTTCAT	AACCACCCAAAGTTCCAAGT	50.0	45.0	58.2	57.5	322 bp	1.0	75.5
psbN-psbT_p5	TGGTTATGCCTTGTACACCG	CGCTATTTCTTCCCGGGAAC	50.0	55.0	57.9	58.7	295 bp	1.0	75.2