Hotspot

trnF-GAA

LSC · 50870-50942 · 73 bp

Back to Huodendron

Pi 0.0137

MarkerSeek score 60.40

Primer available yes

Label trnF-GAA

Feature Metadata

label_name: trnF-GAA
feature_type: tRNA
region: LSC
length_bp: 73
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA_p1	GAGAATGATGCGTCGGGAAT	TCTCTAAACACCTAGATAGATGGC	50.0	41.7	58.1	57.1	213 bp	1.0	40.4
trnF-GAA_p2	GAGAATGATGCGTCGGGAAT	CTCTAAACACCTAGATAGATGGCT	50.0	41.7	58.1	57.1	212 bp	1.0	40.4
trnF-GAA_p3	GAGAATGATGCGTCGGGAAT	AAACACCTAGATAGATGGCT	50.0	40.0	58.1	53.1	208 bp	1.0	40.4
trnF-GAA_p4	CCAGGACCCGGCTAAAATTT	TCTCTAAACACCTAGATAGATGGC	50.0	41.7	58.2	57.1	287 bp	1.0	40.3
trnF-GAA_p5	CCAGGACCCGGCTAAAATTT	CTCTAAACACCTAGATAGATGGCT	50.0	41.7	58.2	57.1	286 bp	1.0	40.3