Hotspot

trnD-GUC

LSC · 32611-32684 · 74 bp

Back to Huodendron

Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnD-GUC

Feature Metadata

label_name: trnD-GUC
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC_p1	TGGTGAGAAACGCGAAAGAA	ATTCGCCAATCTACCACGAG	45.0	50.0	58.1	57.8	294-295 bp	1.0	78.0
trnD-GUC_p2	CTGTGAAATCCTGTCCCCTG	ATTCGCCAATCTACCACGAG	55.0	50.0	57.9	57.8	247 bp	1.0	77.6
trnD-GUC_p3	TGGTGAGAAACGCGAAAGAA	GGCCCAATAATTCGCCAATC	45.0	50.0	58.1	57.6	303-304 bp	1.0	76.3
trnD-GUC_p4	CTGTGAAATCCTGTCCCCTG	GGCCCAATAATTCGCCAATC	55.0	50.0	57.9	57.6	256 bp	1.0	75.8
trnD-GUC_p5	TGGTGAGAAACGCGAAAGAA	CGCCAATCTACCACGAGATG	45.0	55.0	58.1	58.5	291-292 bp	1.0	75.7