Hotspot

rpl23

IRb · 89290-89571 · 282 bp

Back to Huodendron

Pi 0
MarkerSeek score 33.50
Primer available yes
Label rpl23

Feature Metadata

label_name
rpl23
feature_type
gene
region
IRb
length_bp
282
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23_p1 TTTCACTTGACTGCCTACGG ACATAACGAATTGGTGTGGT 50.0 40.0 57.8 55.3 468 bp 1.0 56.8
rpl23_p2 GTGTGCTCGGGTTAGAAGTT ACATAACGAATTGGTGTGGT 50.0 40.0 57.8 55.3 439 bp 1.0 56.8
rpl23_p3 CACTTGACTGCCTACGGTTC ACATAACGAATTGGTGTGGT 55.0 40.0 58.6 55.3 465 bp 1.0 53.6
rpl23_p4 TTTCACTTGACTGCCTACGG CCATACATAACGAATTGGTGTGG 50.0 43.5 57.8 57.8 472 bp 1.0 53.3
rpl23_p5 GTGTGCTCGGGTTAGAAGTT CCATACATAACGAATTGGTGTGG 50.0 43.5 57.8 57.8 443 bp 1.0 53.3