Hotspot

psbA

LSC · 491-1552 · 1062 bp

Back to Huodendron

Pi 0

MarkerSeek score 29.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AAAGGAGCAATAACGCCCTC	AAATTCGTGTGCTTGGGAGT	50.0	45.0	58.2	58.0	1209 bp	1.0	77.9
psbA_p2	AGCAATAACGCCCTCTTCTC	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.7	58.0	1204 bp	1.0	77.3
psbA_p3	GAGCAATAACGCCCTCTTCT	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.7	58.0	1205 bp	1.0	77.3
psbA_p4	GCAATAACGCCCTCTTCTCT	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.7	58.0	1203 bp	1.0	77.3
psbA_p5	GGAGCAATAACGCCCTCTTC	AAATTCGTGTGCTTGGGAGT	55.0	45.0	58.7	58.0	1206 bp	1.0	74.3