Hotspot

ndhF-rpl32

SSC · 115875-116836 · 962 bp

Back to Huodendron

Pi 0.00208
MarkerSeek score 59.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
962
variable_sites
2
pi
0.00208
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAAAGGTATGATCCACGCA AGCTGCCCAATATCCTTTCT 42.9 45.0 57.9 56.6 1087-1090 bp 1.0 60.1
ndhF-rpl32_p2 ACAAAAGGTATGATCCACGCA AAGCTGCCCAATATCCTTTCT 42.9 42.9 57.9 57.3 1088-1091 bp 1.0 58.2
ndhF-rpl32_p3 ACAAAAGGTATGATCCACGCA AGCTGCCCAATATCCTTTCTT 42.9 42.9 57.9 57.3 1087-1090 bp 1.0 58.2
ndhF-rpl32_p4 ACAAAAGGTATGATCCACGCA AAGCTGCCCAATATCCTTTCTT 42.9 40.9 57.9 58.0 1088-1091 bp 1.0 55.5
ndhF-rpl32_p5 ACAAAAGGTATGATCCACGCA AGCTGCCCAATATCCTTTCTTT 42.9 40.9 57.9 58.0 1087-1090 bp 1.0 55.5