Hotspot

rps7-rps12

IRb · 100832-100884 · 53 bp

Back to Huodendron

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TCTTTTGCAGTACCTCGACG	CTGTGGAAAGCCGTATTCGA	50.0	50.0	57.9	58.0	184 bp	1.0	79.9
rps7-rps12_p2	TCTTTTGCAGTACCTCGACG	TCTGTGGAAAGCCGTATTCG	50.0	50.0	57.9	58.0	185 bp	1.0	79.9
rps7-rps12_p3	CTTTTGCAGTACCTCGACGT	CTGTGGAAAGCCGTATTCGA	50.0	50.0	58.2	58.0	183 bp	1.0	78.6
rps7-rps12_p4	CTTTTGCAGTACCTCGACGT	TCTGTGGAAAGCCGTATTCG	50.0	50.0	58.2	58.0	184 bp	1.0	78.6
rps7-rps12_p5	TCTTTTGCAGTACCTCGACG	GGCTTGGAGGGAGATCTTTC	50.0	55.0	57.9	57.7	148 bp	1.0	77.3