Hotspot

accD-psaI

LSC · 62620-63394 · 775 bp

Back to Huodendron

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	879 bp	1.0	73.8
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.8	57.9	885 bp	1.0	73.2
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	52.6	45.0	58.1	58.0	879 bp	1.0	71.3
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.1	58.0	877 bp	1.0	71.3
accD-psaI_p5	TTGATCTAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.9	58.0	934 bp	1.0	70.7