Hotspot

trnC-GCA-petN

LSC · 29662-30233 · 572 bp

Back to Huodendron

Pi 0.00175

MarkerSeek score 59.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 572
variable_sites: 1
pi: 0.00175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.4	58.3	655 bp	1.0	75.2
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	717 bp	1.0	74.5
trnC-GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.5	58.3	630 bp	1.0	74.2
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.5	57.6	692 bp	1.0	73.5
trnC-GCA-petN_p5	TATCCTTTTGGCGACATGGC	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.6	58.3	688 bp	1.0	73.1