Hotspot

rpoC2

LSC · 16255-20451 · 4197 bp

Back to Humulus

Pi 0.00238

MarkerSeek score 55.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4197
variable_sites: 10
pi: 0.00238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGGTGTGGTCCATGTTTGAC	ACTTGACAATTTCACACAGCC	50.0	42.9	58.0	57.3	234 bp	1.0	67.2
rpoC2_p2	GGTGTGGTCCATGTTTGACT	ACTTGACAATTTCACACAGCC	50.0	42.9	58.0	57.3	233 bp	1.0	67.2
rpoC2_p3	CCCGATTTTAGGTGTGGTCC	ACTTGACAATTTCACACAGCC	55.0	42.9	58.3	57.3	243 bp	1.0	65.2
rpoC2_p4	AGGTGTGGTCCATGTTTGAC	CTTGACAATTTCACACAGCCT	50.0	42.9	58.0	57.0	233 bp	1.0	64.9
rpoC2_p5	GGTGTGGTCCATGTTTGACT	CTTGACAATTTCACACAGCCT	50.0	42.9	58.0	57.0	232 bp	1.0	64.9