Hotspot

rpl32-trnL-UAG

SSC · 112673-113476 · 804 bp

Back to Humulus

Pi 0.0118
MarkerSeek score 62.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
804
variable_sites
9
pi
0.0118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAAAGGATATTGGGCAGCA AGCGTGTCTACCGATTTCAC 45.0 50.0 56.9 58.0 887-928 bp 1.0 75.3
rpl32-trnL-UAG_p2 AGAAAGGATATTGGGCAGCA CAGCGTGTCTACCGATTTCA 45.0 50.0 56.9 58.0 888-929 bp 1.0 75.3
rpl32-trnL-UAG_p3 AGAAAGGATATTGGGCAGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 56.9 57.9 897-938 bp 1.0 74.3
rpl32-trnL-UAG_p4 AGAAAGGATATTGGGCAGCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 56.9 57.8 896-937 bp 1.0 73.8
rpl32-trnL-UAG_p5 AGAAAGGATATTGGGCAGCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 56.9 57.8 895-936 bp 1.0 73.8