Hotspot

psbI-trnS-GCU

LSC · 7968-8114 · 147 bp

Back to Humulus

Pi 0.0136

MarkerSeek score 60.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 2
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	260 bp	1.0	80.0
psbI-trnS-GCU_p2	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	261 bp	1.0	80.0
psbI-trnS-GCU_p3	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	261 bp	1.0	80.0
psbI-trnS-GCU_p4	TATCTAATGATCCGGGGCGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	262 bp	1.0	80.0
psbI-trnS-GCU_p5	ATCTAATGATCCGGGGCGTA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	58.1	58.2	223 bp	1.0	78.4