Hotspot

clpP

LSC · 76240-78499 · 2260 bp

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Pi 0.0149
MarkerSeek score 65.60
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2260
variable_sites
209
pi
0.0149
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 ACATGTTCCTCGACGTTGAG CAATACGCAACGGGGACTAA 50.0 50.0 57.9 58.0 2588-2836 bp 1.0 90.2
clpP_p2 TGCACCAACGGAAACCATAA CAATACGCAACGGGGACTAA 45.0 50.0 58.0 58.0 2788-2977 bp 0.925926 86.7
clpP_p3 TTGCACCAACGGAAACCATA CAATACGCAACGGGGACTAA 45.0 50.0 58.0 58.0 2789-2978 bp 0.925926 86.7
clpP_p4 TGAGATTTGCACCAACGGAA CAATACGCAACGGGGACTAA 45.0 50.0 58.0 58.0 2795-2984 bp 0.925926 86.7
clpP_p5 TTGAGATTTGCACCAACGGA CAATACGCAACGGGGACTAA 45.0 50.0 58.0 58.0 2796-2985 bp 0.925926 86.7