Hotspot

rbcL-accD

LSC · 62870-63693 · 824 bp

Back to Hoya

Pi 0.0208
MarkerSeek score 70.20
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
824
variable_sites
111
pi
0.0208
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGGCTAGCAAATGGAGTCC GAATTAAACCACCAGTTTTTCA 55.0 31.8 58.0 53.4 937-1315 bp 1.0 52.0
rbcL-accD_p2 GCTGCTTGTGAGGTATGGAA GAATTAAACCACCAGTTTTTCA 50.0 31.8 57.9 53.4 907-1285 bp 1.0 52.0
rbcL-accD_p3 GGCTAGCAAATGGAGTCCTG GAATTAAACCACCAGTTTTTCA 55.0 31.8 58.3 53.4 935-1313 bp 1.0 52.0
rbcL-accD_p4 GTGAGGCTAGCAAATGGAGT GAATTAAACCACCAGTTTTTCA 50.0 31.8 57.6 53.4 939-1317 bp 1.0 52.0
rbcL-accD_p5 AAATGGAGTCCTGAACTGGC GAATTAAACCACCAGTTTTTCA 50.0 31.8 57.8 53.4 928-1306 bp 0.962963 50.5