Hotspot

atpB-rbcL

LSC · 60679-61441 · 763 bp

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Pi 0.0196
MarkerSeek score 70.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
763
variable_sites
57
pi
0.0196
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TTTCAAGCGTGTAAACCCCA AACACCGGCTTTGAATCCAA 45.0 45.0 57.9 58.3 824-863 bp 1.0 86.8
atpB-rbcL_p2 GGCCGATGATTTGGACGATA AACACCGGCTTTGAATCCAA 50.0 45.0 57.8 58.3 860-899 bp 1.0 85.8
atpB-rbcL_p3 AGGCTACATCTAGTACCGGG AACACCGGCTTTGAATCCAA 55.0 45.0 57.7 58.3 878-917 bp 1.0 84.7
atpB-rbcL_p4 TTTCAAGCGTGTAAACCCCA TAACACCGGCTTTGAATCCA 45.0 45.0 57.9 57.4 825-864 bp 1.0 84.6
atpB-rbcL_p5 ATGATTTGGACGATACGCCC AACACCGGCTTTGAATCCAA 50.0 45.0 58.1 58.3 855-894 bp 0.962963 84.4