Hotspot

trnT-UGU-trnL-UAA

LSC · 52018-52834 · 817 bp

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Pi 0.0117
MarkerSeek score 79.20
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
817
variable_sites
70
pi
0.0117
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 853-912 bp 1.0 87.4
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 885-944 bp 1.0 86.3
trnT-UGU-trnL-UAA_p3 TAGGAAAAAGCCGGCTATCG CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 904-963 bp 1.0 86.2
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 847-906 bp 1.0 86.1
trnT-UGU-trnL-UAA_p5 TAGGAAAAAGCCGGCTATCG CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 898-957 bp 1.0 84.9