Hotspot

trnC-GCA-petN

LSC · 32773-33679 · 907 bp

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Pi 0.0106
MarkerSeek score 73.50
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
907
variable_sites
80
pi
0.0106
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 993-1033 bp 1.0 89.8
trnC-GCA-petN_p2 AGAAAGTTCCGTACTGCGTC ACTTCTTCCCCACACTACGA 50.0 50.0 57.9 58.0 1074-1114 bp 1.0 89.7
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CACTACGAGCGAAAGGGAAA 50.0 50.0 58.1 57.9 981-1021 bp 1.0 89.3
trnC-GCA-petN_p4 AGAAAGTTCCGTACTGCGTC CACTACGAGCGAAAGGGAAA 50.0 50.0 57.9 57.9 1062-1102 bp 1.0 89.2
trnC-GCA-petN_p5 AGAAAGTTCCGTACTGCGTC CACTTCTTCCCCACACTACG 50.0 55.0 57.9 57.9 1075-1115 bp 1.0 89.1