Hotspot

atpH-atpI

LSC · 13823-14940 · 1118 bp

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Pi 0.0102
MarkerSeek score 73.60
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1118
variable_sites
82
pi
0.0102
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT CGCGGCTTATATCGGTGAAT 45.0 50.0 58.0 58.3 1095-1264 bp 1.0 87.0
atpH-atpI_p2 GCGATACCCTCTACAGCTTG CGCGGCTTATATCGGTGAAT 55.0 50.0 57.9 58.3 1182-1351 bp 1.0 85.9
atpH-atpI_p3 AATAACGGAAGCGGCAGAAA CGCGGCTTATATCGGTGAAT 45.0 50.0 58.2 58.3 1096-1265 bp 1.0 85.8
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.0 58.5 1111-1280 bp 1.0 85.1
atpH-atpI_p5 TACCCTCTACAGCTTGACCC CGCGGCTTATATCGGTGAAT 55.0 50.0 58.1 58.3 1178-1347 bp 0.962963 84.3