Hotspot

trnK-UUU-rps16

LSC · 4252-5050 · 799 bp

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Pi 0.0195
MarkerSeek score 69.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
799
variable_sites
97
pi
0.0195
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GCTCGTTGCTTTGTCTTTCC GCCGTACGAGGAGAAAACTT 50.0 50.0 58.0 57.9 1335-1508 bp 1.0 90.9
trnK-UUU-rps16_p2 TCAGGATCAGTCGTGGTCTT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.1 57.9 1144-1315 bp 1.0 90.8
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1149-1269 bp 0.962963 89.2
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1148-1268 bp 0.962963 89.2
trnK-UUU-rps16_p5 TCCCTTTCAGGATCAGTCGT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.1 57.9 1150-1270 bp 0.962963 88.8