Hotspot

rpl2

IRb · 24865-26385 · 1521 bp

Back to Hovenia

Pi NA

MarkerSeek score 32.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRb
length_bp: 1521
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 32.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TGATCATTACGCTTCACCCG	ACGTGTCACAATTAATTACTCCT	50.0	34.8	58.1	56.0	1657 bp	1.0	44.0
rpl2_p2	TGATCATTACGCTTCACCCG	AATGATTCGCTACAAAAGGA	50.0	35.0	58.1	52.9	1691 bp	1.0	44.0
rpl2_p3	TGATCATTACGCTTCACCCG	TGATTCGCTACAAAAGGATT	50.0	35.0	58.1	52.9	1689 bp	1.0	44.0
rpl2_p4	TGATCATTACGCTTCACCCG	TGAACGTGTCACAATTAATTACTCC	50.0	36.0	58.1	57.9	1660 bp	1.0	44.0
rpl2_p5	TGATCATTACGCTTCACCCG	TGATTCGCTACAAAAGGATTT	50.0	33.3	58.1	53.8	1689 bp	1.0	44.0