Hotspot

ndhA

SSC · 152722-155106 · 2385 bp

Back to Hovenia

Pi 0.00196

MarkerSeek score 59.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2385
variable_sites: 7
pi: 0.00196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	47.6	50.0	58.1	58.5	2598-2609 bp	1.0	68.3
ndhA_p2	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAAT	47.6	50.0	57.8	58.5	2601-2612 bp	1.0	67.2
ndhA_p3	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAA	47.6	52.6	58.1	58.0	2598-2609 bp	1.0	64.1
ndhA_p4	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAA	47.6	52.6	57.8	58.0	2601-2612 bp	1.0	63.1
ndhA_p5	CCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	50.0	50.0	56.2	58.5	2597-2608 bp	1.0	62.6