Hotspot

accD

LSC · 88082-89797 · 1716 bp

Back to Hovenia

Pi 0.00155

MarkerSeek score 32.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1716
variable_sites: 4
pi: 0.00155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CGTTTCGGATCATGGATCGA	TTTCCCTTCGCGCAAAATTC	50.0	45.0	57.9	57.9	2569-2609 bp	1.0	79.6
accD_p2	CGTTTCGGATCATGGATCGA	CTGGGGGTTCTTGAGGGATA	50.0	55.0	57.9	58.1	2511-2551 bp	1.0	79.2
accD_p3	CGTTTCGGATCATGGATCGA	CCCTTGCCTAATTTCACGGA	50.0	50.0	57.9	57.9	2474-2514 bp	1.0	79.1
accD_p4	CGTTTCGGATCATGGATCGA	GGACCCCCTTGCCTAATTTC	50.0	55.0	57.9	58.2	2479-2519 bp	1.0	78.3
accD_p5	CGTTTCGGATCATGGATCGA	ACCCCCTTGCCTAATTTCAC	50.0	50.0	57.9	57.8	2477-2517 bp	1.0	78.3