Hotspot

psbE-petL

LSC · 95631-97061 · 1431 bp

Back to Hovenia

Pi 0.0028

MarkerSeek score 47.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1431
variable_sites: 6
pi: 0.0028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1577-1578 bp	1.0	66.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1500-1501 bp	1.0	65.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1586-1587 bp	1.0	65.4
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1591-1592 bp	1.0	65.3
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1585-1586 bp	1.0	65.3