Hotspot

atpB-rbcL

LSC · 85012-85893 · 882 bp

Back to Hovenia

Pi 0.00302

MarkerSeek score 48.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 4
pi: 0.00302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AATACGCCCCAGGTTGTTTT	AACACCGGCTTTGAATCCAA	45.0	45.0	58.0	58.3	1002-1041 bp	1.0	77.9
atpB-rbcL_p2	AATACGCCCCAGGTTGTTTT	ACCGGCTTTGAATCCAACAA	45.0	45.0	58.0	58.3	999-1038 bp	1.0	77.9
atpB-rbcL_p3	ATACGCCCCAGGTTGTTTTT	AACACCGGCTTTGAATCCAA	45.0	45.0	58.0	58.3	1001-1040 bp	1.0	77.9
atpB-rbcL_p4	ATACGCCCCAGGTTGTTTTT	ACCGGCTTTGAATCCAACAA	45.0	45.0	58.0	58.3	998-1037 bp	1.0	77.9
atpB-rbcL_p5	CGGTGGAAAAGCTACATCCA	ACCGGCTTTGAATCCAACAA	50.0	45.0	57.9	58.3	1041-1080 bp	1.0	77.0