Hotspot

trnF-GAA-ndhJ

LSC · 78888-79579 · 692 bp

Back to Hovenia

Pi 0.00289

MarkerSeek score 62.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 3
pi: 0.00289
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	843-844 bp	1.0	78.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	807-808 bp	1.0	78.5
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	831-832 bp	1.0	77.8
trnF-GAA-ndhJ_p4	GTCGTGAATGGTCGGGATAG	ATGCCGGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	856-857 bp	1.0	77.7
trnF-GAA-ndhJ_p5	GAGGATGATGCGTCGTGAAT	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	867-868 bp	1.0	77.6