Hotspot

atpH-atpI

LSC · 41789-42857 · 1069 bp

Back to Hovenia

Pi 0.00374

MarkerSeek score 49.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 6
pi: 0.00374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1157-1161 bp	1.0	80.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1123-1127 bp	1.0	80.5
atpH-atpI_p3	GATCCCTTCTACAGCTTGGC	GCGAATCCATGGAAGGTCAT	55.0	50.0	58.0	58.0	1208-1212 bp	1.0	80.5
atpH-atpI_p4	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	50.0	45.0	58.0	57.9	1187-1191 bp	1.0	79.7
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.0	57.9	1153-1157 bp	1.0	79.7